Western blotting might seem easy at the surface, but there are some small mistakes that can ruin the results. Protein ladders are used in Indian research laboratories, diagnostic training centers, and biotech institutes to accurately read blots. A pre-stained ladder is identical to a visible roadmap during SDS-PAGE and membrane transfers. Colored bands can be seen moving, shifting or transferring in real time. This saves reagents, samples and time, particularly if expensive antibodies are used. It also makes documentation simpler for lab work, which follows SOP...
